All lanes : Anti-GLIPR2 antibody (ab122059) at 1/1000 dilutionLane 1 : Mouse cell lysateLane 2 : Human cell lysateLane 3 : Human cell lysateLysates/proteins at 20000000 cells per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 17 kDa (why is the actual band size different from the predicted?)Exposure time : 2 minutesThis image is courtesy of an anonymous AbreviewSee Abreview
All lanes : Anti-GLIPR2 antibody (ab122059) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : GLIPR2-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))developed using the ECL technique
ab121326, at 1/15, staining GLIPR2 in paraffin embedded Human lung tissue by Immunohistochemistry.