All lanes : Anti-GLO1 antibody (ab52835) at 1/1000 dilutionLane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigenLane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)Lysates/proteins at 20 µg per lane.SecondaryLane 1 : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilutionLane 2 : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution
ab52835 staining human ovarian cancer sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab52835 at 1/50 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.See Abreview