Anti-GLO1 antibody [6F10]
| Name | Anti-GLO1 antibody [6F10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab81461 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 6F10 |
| Applications | ELISA ICC/IF ICC/IF IHC-F WB FC IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant full length Mouse GLO1 protein with a tag |
| Description | Rat Monoclonal |
| Gene | GLO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent staining of HeLa cells with ab81461.
![All lanes : Anti-GLO1 antibody [6F10] (ab81461)Lane 1 : COS-1 (simian) whole cell extractLane 2 : L929 (mouse) whole cell extractLane 3 : HeLa (human) whole cell extract](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/24843_GLO1-Primary-antibodies-ab81461-2.jpg)
All lanes : Anti-GLO1 antibody [6F10] (ab81461)Lane 1 : COS-1 (simian) whole cell extractLane 2 : L929 (mouse) whole cell extractLane 3 : HeLa (human) whole cell extract
ab81461 staining human ovarian carcinoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab81461 at 1/500 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rat polyclonal antibody at 1/200 was used as the secondary antibody.See Abreview
![Overlay histogram showing HeLa cells stained with ab81461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81461, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/24845_GLO1-Primary-antibodies-ab81461-6.jpg)
Overlay histogram showing HeLa cells stained with ab81461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81461, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
A high-salt diet further impairs age-associated declines in cognitive, - A high-salt diet further impairs age-associated declines in cognitive,
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Deletion of the receptor for advanced glycation end products reduces - Deletion of the receptor for advanced glycation end products reduces
Reiniger N, Lau K, McCalla D, Eby B, Cheng B, Lu Y, Qu W, Quadri N, Ananthakrishnan R, Furmansky M, Rosario R, Song F, Rai V, Weinberg A, Friedman R, Ramasamy R, D'Agati V, Schmidt AM. Diabetes. 2010 Aug;59(8):2043-54.