Anti-GLP1R antibody

• Supplier: Abcam
• Catalog: ab39072
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-F WB IHC-P
• Species Reactivities: Mouse, Rat, Dog, Human, Sheep
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human GLP1R
• Blocking Peptide: Human GLP1R peptide
• Description: Rabbit Polyclonal
• Gene: GLP1R
• Conjugate: Unconjugated

Product images

All lanes : Anti-GLP1R antibody (ab39072) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Rat) Tissue Lysate Lane 3 : Brain (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of ab39072 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39072, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of GLP1R staining in rat pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39072, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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A novel humanized GLP-1 receptor model enables both affinity purification and - A novel humanized GLP-1 receptor model enables both affinity purification and

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GLP-1 receptor activation indirectly reduces hepatic lipid accumulation but does - GLP-1 receptor activation indirectly reduces hepatic lipid accumulation but does

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Pulmonary GLP-1 receptor increases at birth and exogenous GLP-1 receptor agonists - Pulmonary GLP-1 receptor increases at birth and exogenous GLP-1 receptor agonists

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The identification of novel proteins that interact with the GLP-1 receptor and - The identification of novel proteins that interact with the GLP-1 receptor and

Huang X, Dai FF, Gaisano G, Giglou K, Han J, Zhang M, Kittanakom S, Wong V, Wei L, Showalter AD, Sloop KW, Stagljar I, Wheeler MB. Mol Endocrinol. 2013 Sep;27(9):1550-63.

Glucagon like peptide-1 receptor expression in the human thyroid gland. - Glucagon like peptide-1 receptor expression in the human thyroid gland.

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