Flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling GLP2R with ab181030 at 1/220 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
All lanes : Anti-GLP2R antibody [EPR12812] (ab181030) at 1/5000 dilutionLane 1 : 293 lysateLane 2 : A549 lysateLane 3 : HepG2 lysateLane 4 : HeLa lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique