Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm and endoplasmic reticulum. Recommended concentration of ab122192 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
ab122192 at 1/350 dilution staining GLT25D2 in Paraffin-embedded Human Breast tissue by Immunohistochemistry.
All lanes : Anti-GLT25D2 antibody (ab122192) at 1/250 dilutionLane 1 : RT 4 lysateLane 2 : U 51 MG lysateLane 3 : Human plasma lysateLane 4 : Human liver lysateLane 5 : Human tonsil lysatedeveloped using the ECL technique