Anti-Glucagon Receptor antibody
| Name | Anti-Glucagon Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab75240 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthesized peptide derived from internal of human Glucagon Receptor |
| Blocking Peptide | Glucagon Receptor peptide |
| Description | Rabbit Polyclonal |
| Gene | GCGR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Glucagon Receptor antibody (ab75240) at 1/500 dilutionLane 1 : extracts from COLO205 cellsLane 2 : extracts from COLO205 cells with immunizing peptide at 5 µgLysates/proteins at 15 µg per lane.
Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75240. Right panel: same, after pre-incubation with the immunizing peptide.
IHC image of ab75240 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75240, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.