Anti-Glucose 6 phosphate isomerase antibody
| Name | Anti-Glucose 6 phosphate isomerase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab68643 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Pig, Chimpanzee, Monkey, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Glucose 6 phosphate isomerase |
| Description | Rabbit Polyclonal |
| Gene | GPI |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab68643 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68643, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293 and MCF7 cells at 5µg/ml.
All lanes : Anti-Glucose 6 phosphate isomerase antibody (ab68643) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.