ab33245 (1/200) staining GLUT10 in mice dissected inner ear tissues. Used in combination with an anti-rabbit secondary antibody (1/400) conjugated to Cy3®. Cochlear sections show strong immunoreactivity (stained red) in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate stainning.
All lanes : Anti-Glucose Transporter GLUT10 antibody (ab33245) at 1/500 dilutionLane 1 : 10ug of mouse liver protein extractLane 2 : 10ug of cochlea protein extract from E18 mouseLane 3 : 10ug of cochlea protein extract from P7 mouseLane 4 : 10ug of cochlea protein extract from P10 mouseLane 5 : 10ug of cochlea protein extract from P14 mouseLane 6 : 10ug of cochlea protein extract from P17 mouseLane 7 : 10ug of cochlea protein extract from P60 mouseLane 8 : 10ug of cochlea protein extract from a 1 year old mouseSecondaryAnti-rabbit IgG conjugated to HRP at 1/3000 dilution