Anti-Glucose Transporter GLUT2 antibody [5D1]
| Name | Anti-Glucose Transporter GLUT2 antibody [5D1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab85715 |
| Prices | $385.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 5D1 |
| Applications | IHC-P ICC/IF FC IHC-F WB |
| Species Reactivities | Human |
| Antigen | Human Glucose Transporter GLUT2 transfected NS0 cells |
| Description | Mouse Monoclonal |
| Gene | SLC2A2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab85715, at 10 µg/ml, staining Glucose Transporter GLUT2 in formalin-fixed, paraffin-embedded human Liver tissue by Immunohistochemistry.
![Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715) at 1 µg/ml + Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/25211_Glucose-Transporter-GLUT2-Primary-antibodies-ab85715-4.jpg)
Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715) at 1 µg/ml + Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing HepG2 cells stained with ab85715 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85715, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/25212_Glucose-Transporter-GLUT2-Primary-antibodies-ab85715-5.jpg)
Overlay histogram showing HepG2 cells stained with ab85715 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85715, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunohistochemical analysis of frozen pancreatic tissue in control (left) or TAT-MafA mutant (right) neonate mice. Glucose Transporter GLUT2 was stained using ab85715 (green), at 10 mg/ml. An AlexaFluor®647-conjugated donkey anti-mouse IgG was used as the secondary antibody.
Product References
Correlation between biological marker expression and fluorine-18 - Correlation between biological marker expression and fluorine-18
Mano Y, Aishima S, Kubo Y, Tanaka Y, Motomura T, Toshima T, Shirabe K, Baba S, Maehara Y, Oda Y. Am J Clin Pathol. 2014 Sep;142(3):391-7.
Liver-restricted Repin1 deficiency improves whole-body insulin sensitivity, - Liver-restricted Repin1 deficiency improves whole-body insulin sensitivity,
Kern M, Kosacka J, Hesselbarth N, Bruckner J, Heiker JT, Flehmig G, Kloting I, Kovacs P, Matz-Soja M, Gebhardt R, Krohn K, Sales S, Abshagen K, Shevchenko A, Stumvoll M, Bluher M, Kloting N. Diabetes. 2014 Oct;63(10):3295-309.
TAT-mediated transduction of MafA protein in utero results in enhanced pancreatic - TAT-mediated transduction of MafA protein in utero results in enhanced pancreatic
Vargas N, Alvarez-Cubela S, Giraldo JA, Nieto M, Fort NM, Cechin S, Garcia E, Espino-Grosso P, Fraker CA, Ricordi C, Inverardi L, Pastori RL, Dominguez-Bendala J. PLoS One. 2011;6(8):e22364.