Anti-Glutamyl Prolyl tRNA synthetase antibody

• Supplier: Abcam
• Catalog: ab31531
• Prices: $395.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IP IHC-P
• Species Reactivities: Mouse, Human, Pig
• Antigen: V573-K889 native human recombinant EPRS
• Description: Rabbit Polyclonal
• Gene: EPRS
• Conjugate: Unconjugated

Product images

Anti-Glutamyl Prolyl tRNA synthetase antibody (ab31531) at 1/10000 dilution + HEK293 cell line at 30 µg

Ab31531 staining Glutamyl Prolyl tRNA synthetase in Hela cell line by Immunofluorescence.

Anti-Glutamyl Prolyl tRNA synthetase antibody (ab31531) at 1/20000 dilution + whole cell lysate prepared from HEK-293 cells at 50 µg/mlSecondaryGoat anti-rabbit IgG (H+L) at 1/10000 dilution

IHC image of ab31531 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31531, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ab31531 staining Glutamyl Prolyl tRNA synthetase in the Human Liver cell line Huh7 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with Triton X-100 0.5% and blocked with 10% Serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 10% Serum) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Product References

Reinvestigation of aminoacyl-tRNA synthetase core complex by affinity - Reinvestigation of aminoacyl-tRNA synthetase core complex by affinity

Kim K, Park SJ, Na S, Kim JS, Choi H, Kim YK, Paek E, Lee C. PLoS One. 2013 Dec 2;8(12):e81734.

RNA binding targets aminoacyl-tRNA synthetases to translating ribosomes. - RNA binding targets aminoacyl-tRNA synthetases to translating ribosomes.

David A, Netzer N, Strader MB, Das SR, Chen CY, Gibbs J, Pierre P, Bennink JR, Yewdell JW. J Biol Chem. 2011 Jun 10;286(23):20688-700.