All lanes : Anti-Glutamyl Prolyl tRNA synthetase antibody (ab157122) at 0.1 µg/mlLane 1 : 293T whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : TCMK1 whole cell lysateLane 5 : NIH 3T3 whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique
Detection of Glutamyl Prolyl tRNA synthetase by Western Blot of Immunprecipitate. ab157122 at 1 µg/ml labeling Glutamyl Prolyl tRNA synthetase in 293T whole cell lysate immunoprecipitated using ab157122 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds