Anti-Glutathione Peroxidase 1 antibody
| Name | Anti-Glutathione Peroxidase 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab22604 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Glutathione Peroxidase 1 |
| Blocking Peptide | Human Glutathione Peroxidase 1 peptide |
| Description | Rabbit Polyclonal |
| Gene | GPX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Glutathione Peroxidase 1 antibody (ab22604) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Glutathione Peroxidase 1 antibody (ab22604) at 1 µg/mlLane 1 : Human liver normal tissue lysateLane 2 : Liver (Mouse) Tissue Lysate (ab7935)Lane 3 : Human liver normal tissue lysate with Human Glutathione Peroxidase 1 peptide (ab25301) at 1 µg/mlLane 4 : Liver (Mouse) Tissue Lysate (ab7935) with Human Glutathione Peroxidase 1 peptide (ab25301) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPerformed under reducing conditions.
Image courtesy of Human Protein Atlasab22604 staining Glutathione Peroxidase 1 in Human kidney. The paraffin embedded human kidney tissue was incubated with ab22604 (1/375 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab22604 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab22604 stained human HepG2 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab22604, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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