Anti-Glutathione Peroxidase 1 antibody
| Name | Anti-Glutathione Peroxidase 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59546 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IHC-F IHC-P |
| Species Reactivities | Rat, Human, Mouse, Bovine, Pig, Chimpanzee, Monkey, Orangutan |
| Antigen | Synthetic peptide corresponding to Human Glutathione Peroxidase 1 aa 175-194 |
| Description | Rabbit Polyclonal |
| Gene | GPX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab59546, at a 1/100 dilution, staining rat Cellular Glutathione Peroxidase in brain neurons using Immunohistochemistry. Secondary antibody donkey anti-rabbit conjugated to Cy3 at a 1/100 dilution.
Anti-Glutathione Peroxidase 1 antibody (ab59546) at 1/2000 dilution + Liver (Mouse) Tissue Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionObserved band size : 22 kDa (why is the actual band size different from the predicted?)Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab59546 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59546, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab59546 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59546, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
gamma-Glutamylcysteine detoxifies reactive oxygen species by acting as - gamma-Glutamylcysteine detoxifies reactive oxygen species by acting as
Quintana-Cabrera R, Fernandez-Fernandez S, Bobo-Jimenez V, Escobar J, Sastre J, Almeida A, Bolanos JP. Nat Commun. 2012 Mar 6;3:718.
The neurogenic basic helix-loop-helix transcription factor NeuroD6 confers - The neurogenic basic helix-loop-helix transcription factor NeuroD6 confers
Uittenbogaard M, Baxter KK, Chiaramello A. ASN Neuro. 2010 May 24;2(2):e00034.