Anti-Glutathione S Transferase pi antibody - C-terminal (ab135535) at 1/50 dilution + Y79 cell line lysate at 35 µg
All lanes : Anti-Glutathione S Transferase pi antibody - C-terminal (ab135535) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the Glutathione S Transferase pi geneLysates/proteins at 2 µg per lane.
Flow cytometric analysis of widr cells labelling Glutathione S Transferase pi with ab135535 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-Glutathione S Transferase pi antibody - C-terminal (ab135535) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the Glutathione S Transferase pi geneLysates/proteins at 2 µg per lane.
Immunofluorescent analysis of HeLa cells labelling Glutathione S Transferase pi with ab135535 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).