All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilutionLane 1 : Mouse adipose tissue lysateLane 2 : Mouse adipose tissue lysateLysates/proteins at 25 µg per lane.SecondaryHRP-conjugated Donkey anti-rabbit polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilutionLane 1 : Fetal liver lysateLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
ab126599, at 1/100 dilution, staining Glycerol kinase in permeabilized HepG2 cells by Immunofluorescence (red). A rabbit IgG is used as a negative control (green).
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD