Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y]

• Supplier: Abcam
• Catalog: ab81230
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP852Y
• Applications: WB IHC-P ICC/IF IP
• Species Reactivities: Mouse, Human
• Antigen: Synthetic phosphopeptide corresponding to residues surrounding Ser641 of Human Glycogen synthase 1
• Description: Rabbit Monoclonal
• Gene: GYS1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] (ab81230) at 1/10000 dilutionLane 1 : Mouse muscle lysate, untreatedLane 2 : Mouse muscle lysate, treated with APLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution

Immunohistochemical analysis of Human liver tissue, using 1/100 ab81230

ICC/IF image of ab81230 stained A431 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81230, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Differential activity of GSK-3 isoforms regulates NF-kappaB and TRAIL- or - Differential activity of GSK-3 isoforms regulates NF-kappaB and TRAIL- or

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Myocardial glycophagy - a specific glycogen handling response to metabolic stress - Myocardial glycophagy - a specific glycogen handling response to metabolic stress

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Fasting-induced protein phosphatase 1 regulatory subunit contributes to - Fasting-induced protein phosphatase 1 regulatory subunit contributes to

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