Performed under reducing conditions.Observed band size : 17 kDa (why is the actual band size different from the predicted?)Western blot analysis of recombinant GM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.
Performed under non-reducing conditions.Observed band size : 17 kDa (why is the actual band size different from the predicted?)Western blot analysis of recombinant GM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.
Less than 0.10 ug/ml ab9741 will neutralize 50% of the bioactivity of 1 ng/ml m-GM-CSF.
IHC image of GM-CSF staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9741, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.