All lanes : Anti-GM-CSF antibody (ab77768) at 1 µg/mlLane 1 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate Lane 4 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate Lane 5 : HL60 (Human promyelocytic leukemia cell line) Whole Cell LysateLane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.