Anti-GM130 antibody (ab169276) at 1 µg/ml + Human placenta tissue lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
All lanes : Anti-GM130 antibody (ab169276) at 1 µg/mlLane 1 : GM130 transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
Immunofluorescent analysis of permeabilized HeLa cells labeling GM130 with ab169276 at 7µg/ml.