Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

• Supplier: Abcam
• Catalog: ab52649
• Prices: $404.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP892Y
• Applications: ICC/IF ICC/IF IHC-P ICC/IF IHC-F WB IP
• Species Reactivities: Mouse, Rat, Bovine, Dog, Human, Monkey, Monkey
• Antigen: A synthetic peptide corresponding to residues near the N-term of human GM130
• Description: Rabbit Monoclonal
• Gene: GOLGA2
• Conjugate: Unconjugated

Product images

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/200000 dilution + 10 µg of HeLa cell lysateSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

ab52649 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.ab52649 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.

ab52649 staining GM130 (magenta) in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. Nuclei stained with Picogreen.See Abreview

ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.See Abreview

ab52649 staining GM130 in Bovine brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 90 minutes at 37°C. Samples were incubated with primary antibody (1/100 in 0.1% saponin + 1% BSA ) for 18 hours at 4°C. An undiluted Alexa Fluor®568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

ICC/IF image of ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52946, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF image of ab52649 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Hofmann K, Thiele C, Schott HF, Gaebler A, Schoene M, Kiver Y, Friedrichs S, Lutjohann D, Kuerschner L. J Lipid Res. 2014 Mar;55(3):583-91.

Palmitoylation of the immunity related GTPase, Irgm1: impact on membrane - Palmitoylation of the immunity related GTPase, Irgm1: impact on membrane

Henry SC, Schmidt EA, Fessler MB, Taylor GA. PLoS One. 2014 Apr 21;9(4):e95021.

The Drosophila effector caspase Dcp-1 regulates mitochondrial dynamics and - The Drosophila effector caspase Dcp-1 regulates mitochondrial dynamics and

DeVorkin L, Go NE, Hou YC, Moradian A, Morin GB, Gorski SM. J Cell Biol. 2014 May 26;205(4):477-92.

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Maier PJ, Zemoura K, Acuna MA, Yevenes GE, Zeilhofer HU, Benke D. J Biol Chem. 2014 May 2;289(18):12896-907.

Proinsulin intermolecular interactions during secretory trafficking in pancreatic - Proinsulin intermolecular interactions during secretory trafficking in pancreatic

Haataja L, Snapp E, Wright J, Liu M, Hardy AB, Wheeler MB, Markwardt ML, Rizzo M, Arvan P. J Biol Chem. 2013 Jan 18;288(3):1896-906.

Proteomics analyses of human optic nerve head astrocytes following biomechanical - Proteomics analyses of human optic nerve head astrocytes following biomechanical

Rogers RS, Dharsee M, Ackloo S, Sivak JM, Flanagan JG. Mol Cell Proteomics. 2012 Feb;11(2):M111.012302.

Type XII collagen regulates osteoblast polarity and communication during bone - Type XII collagen regulates osteoblast polarity and communication during bone

Izu Y, Sun M, Zwolanek D, Veit G, Williams V, Cha B, Jepsen KJ, Koch M, Birk DE. J Cell Biol. 2011 Jun 13;193(6):1115-30.

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Schonherr C, Ruuth K, Eriksson T, Yamazaki Y, Ottmann C, Combaret V, Vigny M, Kamaraj S, Palmer RH, Hallberg B. Transl Oncol. 2011 Aug;4(4):258-65. Epub 2011 Aug 1.

Disease-associated missense mutations in bestrophin-1 affect cellular trafficking - Disease-associated missense mutations in bestrophin-1 affect cellular trafficking

Milenkovic VM, Rohrl E, Weber BH, Strauss O. J Cell Sci. 2011 Sep 1;124(Pt 17):2988-96.