All lanes : Anti-GMP Synthase antibody - C-terminal (ab135538) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the GMP Synthase geneLysates/proteins at 2 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labelling GMP Synthase with ab135538 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of HeLa cells labelling GMP Synthase with ab135538 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.