Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling GNB5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Anti-GNB5 antibody [EPR14778] (ab185206) at 1/2000 dilution + Human cerebellum lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of GNB5 in immunoprecipitation pellets from Rat brain lysate using ab185206 at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
Flow cytometry analysis of 2% paraformaldehyde fixed HACAT cells labeling GNB5 with ab185206 at 1/160 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed U87-MG cells labeling GNB5 with ab185206 at 1/100 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstained with Dapi blue.
Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling GNB5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Anti-GNB5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Mouse brain lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-GNB5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Rat brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution