Anti-GOLPH4 antibody [EPR13439] (ab197595) at 1/2500 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-GOLPH4 antibody [EPR13439] (ab197595) at 1/5000 dilutionLane 1 : HepG2(Human liver hepatocellular carcinoma) whole cell lysateLane 2 : 293(Human embryonic kidney) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling GOLPH4 with ab197595 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human renal adenocarcinoma tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.