Flow cytometric analysis of MDA-MB231 cells labeling GPA33 using ab175870 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin fixed and paraffin embedded Human colon carcinoma labeling GPA33 using ab175870 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
All lanes : Anti-GPA33 antibody - C-terminal (ab175870) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : 293 cell lysateLane 3 : MDA-MB231 cell lysateLysates/proteins at 35 µg per lane.