Anti-GPCR GPR101 antibody
| Name | Anti-GPCR GPR101 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab75241 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse |
| Antigen | Synthesized peptide derived from C-terminal region of human GPCR GPR101 |
| Description | Rabbit Polyclonal |
| Gene | GPR101 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-GPCR GPR101 antibody (ab75241) at 1/500 dilutionLane 1 : extracts from COS-7 cellsLane 2 : extracts from COS-7 cells with immunizing peptide at 5 µgLysates/proteins at 15 µg per lane.
Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75241. Right panel: same, after preincubation with the immunizing peptide.
IHC image of ab75241 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75241, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.