Anti-GPCR GPR125 antibody
| Name | Anti-GPCR GPR125 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab51705 |
| Prices | $384.00 |
| Sizes | 250 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA IHC-F IHC-P |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide: TKRGNNTRTVDIVVLESSAQYCPPERV conjugated to KLH, corresponding to internal sequence amino acids 328-354 of Human GPCR GPR125 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | ADGRA3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab51705 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51705, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Characterization of human spermatogonial stem cell markers in fetal, pediatric, - Characterization of human spermatogonial stem cell markers in fetal, pediatric,
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Differential marker protein expression specifies rarefaction zone-containing - Differential marker protein expression specifies rarefaction zone-containing
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TEX11 modulates germ cell proliferation by competing with estrogen receptor beta - TEX11 modulates germ cell proliferation by competing with estrogen receptor beta
Yu YH, Siao FP, Hsu LC, Yen PH. Mol Endocrinol. 2012 Apr;26(4):630-42.
Isolation, characterization, and culture of human spermatogonia. - Isolation, characterization, and culture of human spermatogonia.
He Z, Kokkinaki M, Jiang J, Dobrinski I, Dym M. Biol Reprod. 2010 Feb;82(2):363-72.