All lanes : Anti-GPCR GPR20 antibody (ab75559) at 1/500 dilutionLane 1 : extracts from LOVO cellsLane 2 : extracts from LOVO cells with immunizing peptide at 5 µgLysates/proteins at 20 µg per lane.
Left panel: immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
Left panel: immunofluorescence analysis of A549 cells, using 1/500 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.