All lanes : Anti-GPCR GPR32 antibody (ab79516) at 1/500 dilutionLane 1 : Extracts from A549 cellsLane 2 : Extracts from A549 cells with immunizing peptide at 5 µgLysates/proteins at 15 µg per lane.
Left panel: immunofluorescence analysis of A549 cells using ab79516 at a 1/500 dilution. Right panel: same, after pre-incubation with the immunizing peptide.
ab79516 staining GPCR GPR32 in Human primary small airway epithelial cells by Flow Cytometry. Cells were fixed with formaldehyde and incubated with the primary antibody (1/250) for 30 minutes at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/800) was used as the secondary antibody.Gating Strategy: Forward and side scatter.See Abreview