ICC/IF image of ab72157 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72157 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-GPCR GPR65 antibody (ab72157) at 1 µg/mlLane 1 : Human kidney lysateLane 2 : Human kidney lysate with immunising peptide
ab72157 at 20µg/ml staining GPCR GPR65 in human tonsil by Immunohistochemistry, Paraffin-embedded tissue.