All lanes : Anti-GPCR LOC51210 antibody [EPR14402] - C-terminal (ab185218) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of GPCR LOC51210 in immunoprecipitation pellets from HeLa lysate ab185218 used at 1/50 dilution Lane 1. Lane 2 shows PBS negative control. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000.