ab65423 staining GPCR TAS1R3 in murine arcuate nucleus of the hypothalamus by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and incubated with primary antibody (1/50 in normal donkey serum in PBS containing 0.25% Triton X-100). An AlexaFluor®488-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
ab65423 staining GPCR TAS1R3 in murine N38 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and incubated with primary antibody overnight (1/50 in normal donkey serum in PBS containing 0.25% Triton X-100). An AlexaFluor®488-conjugated donkey anti-rabbit IgG was used as the secondary antibody.