All lanes : Anti-GPD2 antibody [EPR14259] (ab188585) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF-7 cell lysateLane 3 : NIH:OVCAR-3 cell lysateLane 4 : U87-MG cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GPD2 with ab188585 at 1/310 dilution (red) compared to a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Western blot aalysis of GPD2 in HeLa cell lysate immunoprecipitated using ab188585 at 1/50 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.