All lanes : Anti-GPD2 antibody [EPR14260] (ab182144) at 1/2000 dilutionLane 1 : NIH: OVCAR-3 cell lysateLane 2 : MCF-7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human pancreas tissue sections labeling GPD2 using ab182144 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Flow Cytometry analysis of HeLa cells labeling GPD2 using ab182144 at a 1/140 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.