Anti-GPR177 antibody
| Name | Anti-GPR177 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72385 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human GPR177 aa 524-541 (C terminal) |
| Description | Chicken Polyclonal |
| Gene | WLS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab72385 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72385 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-GPR177 antibody (ab72385) at 1/2000 dilutionLane 1 : Molecular weight markersLane 2 : HEK293 cell lysate transfected with GPR177-GFP