All lanes : Anti-GPR91 antibody (ab41505) at 1 µg/mlLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Kidney (Rat) Tissue LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : A498 (Human Kidney Carcinoma) Whole Cell LysateLane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.