ab84571 staining GPSM2 in Dog kidney cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 3% BSA for 30 minutes. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C.See Abreview
ab84571 at 5 µg/ml staining GPSM2 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Anti-GPSM2 antibody (ab84571) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab84571 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84571, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab84571 at 5 µg/ml staining GPSM2 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).