Anti-GRASP65 antibody

• Supplier: Abcam
• Catalog: ab30315
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP WB ICC/IF ICC/IF IHC-P IHC-F
• Species Reactivities: Mouse, Rat, Human, Xenopus
• Antigen: Recombinant full length 6-his tagged protein (Mouse)
• Description: Rabbit Polyclonal
• Gene: GORASP1
• Conjugate: Unconjugated

Product images

GRASP65 antibody (ab30315) labelling in mouse Cor1 cell cells. Cor1 cells were fixed with formaldehyde and permeabilised. 1% BSA used as blocking agent for 10 mins. Primary antibody ab30315 incubated at rt°C at 1/5000 for 2 hours in TBS/BSA/azide/Triton.  Secondary Antibody Name: anti rabbit IgG Alexa Fluor® 594 1/1000. ab30315 in Cor1 cells appears to localise discretely to an intracellular compartment that could be the Golgi Appparatus. This pattern is very similar to that in this publication: http://jcb.rupress.org/cgi/content/full/156/3/411-aSee Abreview

ab30315 staining GRASP65 in rat hippocampal tissue (sub-region) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citric acid pH 6.0. Samples were then blocked with 1% BSA for 10 minutes at room temperature followed by incubation with the primary antibody at a 1/6000 dilution for 16 hours. A biotin-conjugated anti-rabbit was used as secondary antibody at a 1/300 dilution. Nuclei are lightly counterstained with Haemalum.

ab30315, staining GRASP65 (green) in murine hippocampus tissue by Immunohistochemistry (PFA perfusion fixed frozen sections). Secions were incubated with primary antibody and an AlexaFluor®488-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

Product References

Protein kinase LKB1 regulates polarized dendrite formation of adult hippocampal - Protein kinase LKB1 regulates polarized dendrite formation of adult hippocampal

Huang W, She L, Chang XY, Yang RR, Wang L, Ji HB, Jiao JW, Poo MM. Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):469-74.

Ouabain-induced internalization and lysosomal degradation of the Na+/K+-ATPase. - Ouabain-induced internalization and lysosomal degradation of the Na+/K+-ATPase.

Cherniavsky-Lev M, Golani O, Karlish SJ, Garty H. J Biol Chem. 2014 Jan 10;289(2):1049-59.

Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via - Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via

Xia WF, Tang FL, Xiong L, Xiong S, Jung JU, Lee DH, Li XS, Feng X, Mei L, Xiong WC. J Cell Biol. 2013 Mar 18;200(6):821-37.

Identification of Stk25 as a genetic modifier of Tau phosphorylation in - Identification of Stk25 as a genetic modifier of Tau phosphorylation in

Matsuki T, Zaka M, Guerreiro R, van der Brug MP, Cooper JA, Cookson MR, Hardy JA, Howell BW. PLoS One. 2012;7(2):e31152.

Arl5b is a Golgi-localised small G protein involved in the regulation of - Arl5b is a Golgi-localised small G protein involved in the regulation of

Houghton FJ, Bellingham SA, Hill AF, Bourges D, Ang DK, Gemetzis T, Gasnereau I, Gleeson PA. Exp Cell Res. 2012 Mar 10;318(5):464-77.

Intracellular trafficking of FXYD1 (phospholemman) and FXYD7 proteins in Xenopus - Intracellular trafficking of FXYD1 (phospholemman) and FXYD7 proteins in Xenopus

Moshitzky S, Asher C, Garty H. J Biol Chem. 2012 Jun 15;287(25):21130-41.

The amyloid precursor protein copper binding domain histidine residues 149 and - The amyloid precursor protein copper binding domain histidine residues 149 and

Spoerri L, Vella LJ, Pham CL, Barnham KJ, Cappai R. J Biol Chem. 2012 Aug 3;287(32):26840-53.

Isolation of rafts from mouse brain tissue by a detergent-free method. - Isolation of rafts from mouse brain tissue by a detergent-free method.

Persaud-Sawin DA, Lightcap S, Harry GJ. J Lipid Res. 2009 Apr;50(4):759-67.