All lanes : Anti-GRASP65 antibody [EPR12439] - C-terminal (ab174834) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling GRASP65 with ab174834 at 1/50 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, labeling GRASP65 with ab174834 at 1/50 dilution.
Immunofluorescent analysis of HepG2 cells, labeling GRASP65 with ab174834 at 1/100 dilution (red). DAPI nuclear staining (blue).
Flow cytometry analysis of permeabilized HeLa cells labeling GRASP65 with ab174834 at 1/10 dilution (red), or negative control rabbit IgG (green).
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling GRASP65, immunoprecipitated using ab174834 at 1/10 dilution.