Anti-GRIM19 antibody
| Name | Anti-GRIM19 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab134325 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Dog, Pig, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GRIM19 |
| Description | Rabbit Polyclonal |
| Gene | NDUFA13 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab134325 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab134325, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-GRIM19 antibody (ab134325) at 1 µg/ml + Heart (Human) Whole Cell Lysate - fetal normal tissue at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.