ab25963 staining GRIP1 (red) in mouse skeletal (gastrocnemius) muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + Tween-20) for 12 hours at 4°C. A Cy3®-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. DAPI (blue) - Nuclei.See Abreview
All lanes : Anti-GRIP1 antibody (ab25963) at 2 µg/mlLane 1 : Brain (Mouse) Tissue Lysate - postnatal day 7Lane 2 : Brain (Rat) Tissue Lysate - normal adult tissueLane 3 : Brain (Rat) Tissue Lysate - postnatal day 7Lysates/proteins at 15 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Anti-GRIP1 antibody (ab25963) at 2 µg/ml + Brain (Rat) Tissue Lysate - postnatal day 7 at 15 µgSecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.