ab17882 staining GRO alpha in mouse gingiva, junctional epithelium tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 1.5% normal goat serum in PBST for 20 minutes at 37°C. The primary antibody was diluted 1/100 in the blocking solution and incubated with the sample for 20 hours at 4°C. A biotin-conjugated anti-rabbit polyclonal was used as the secondary antibody as part of an ABC procedure, diluted 1/80.See Abreview
ICC/IF image of ab17882 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17882, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.