Anti-Grp75 antibody
| Name | Anti-Grp75 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53098 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide derived from the C-terminus of human GRP75 |
| Description | Rabbit Polyclonal |
| Gene | HSPA9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical analysis of GRP75 in paraffin-embedded human breast carcinoma tissue, using ab53098 at 1/50 dilution, in the presence (right) or absence (left) of peptide.
All lanes : Anti-Grp75 antibody (ab53098) at 1/300 dilutionLane 1 : Cos7 cell extractLane 2 : Cos7 cell extract with peptide
ICC/IF image of ab53098 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53098, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Secretion modification region-derived peptide disrupts HIV-1 Nef's interaction - Secretion modification region-derived peptide disrupts HIV-1 Nef's interaction
Shelton MN, Huang MB, Ali SA, Powell MD, Bond VC. J Virol. 2012 Jan;86(1):406-19.
AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex - AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex
Hammond JC, Meador-Woodruff JH, Haroutunian V, McCullumsmith RE. PLoS One. 2012;7(6):e39190.
Control of hepatic nuclear superoxide production by glucose 6-phosphate - Control of hepatic nuclear superoxide production by glucose 6-phosphate
Spencer NY, Yan Z, Boudreau RL, Zhang Y, Luo M, Li Q, Tian X, Shah AM, Davisson RL, Davidson B, Banfi B, Engelhardt JF. J Biol Chem. 2011 Mar 18;286(11):8977-87.
Effect of endogenous mutant and wild-type PINK1 on Parkin in fibroblasts from - Effect of endogenous mutant and wild-type PINK1 on Parkin in fibroblasts from
Rakovic A, Grunewald A, Seibler P, Ramirez A, Kock N, Orolicki S, Lohmann K, Klein C. Hum Mol Genet. 2010 Aug 15;19(16):3124-37.
Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts. - Mutant Parkin impairs mitochondrial function and morphology in human fibroblasts.
Grunewald A, Voges L, Rakovic A, Kasten M, Vandebona H, Hemmelmann C, Lohmann K, Orolicki S, Ramirez A, Schapira AH, Pramstaller PP, Sue CM, Klein C. PLoS One. 2010 Sep 27;5(9):e12962.