All lanes : Anti-Grp75 antibody [9F8] (ab171089) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : K562 whole cell lysateLane 4 : A431 whole cell lysateLane 5 : COS7 whole cell lysateLane 6 : NIH3T3 whole cell lysateLane 7 : NRK whole cell lysateLysates/proteins at 50 µg per lane.SecondaryGoat anti-Mouse HRP at 1/20000 dilutiondeveloped using the ECL technique
Immunofluorescent analysis of HeLa cells labeling Grp75 with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells.
Detection of Grp75 in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2µg per 750µg lysate. Detected with ab171089 at 1/1000 dilution.