All lanes : Anti-GRPR antibody (ab39883) at 1 µg/mlLane 1 : Brain (Human) Membrane LysateLane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab3950)Lysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ab39883 staining human pancreas tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 45 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit antibody was used as the secondary.See Abreview