Anti-GRPR antibody
| Name | Anti-GRPR antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39963 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P IP |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GRPR |
| Description | Rabbit Polyclonal |
| Gene | GRPR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![GRPR was immunoprecipitated using 0.5mg Caco2 whole cell extract, 5µg of Rabbit polyclonal to GRPR and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Caco2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab39963.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 53kDa; GRPR](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/27989_ab39963-195126-IPV021ab3996320minM.jpg)
GRPR was immunoprecipitated using 0.5mg Caco2 whole cell extract, 5µg of Rabbit polyclonal to GRPR and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Caco2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab39963.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 53kDa; GRPR
All lanes : Anti-GRPR antibody (ab39963) at 1 µg/mlLane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab3950)Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate (ab7910)Lysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Product References
PSMA, EpCAM, VEGF and GRPR as imaging targets in locally recurrent prostate - PSMA, EpCAM, VEGF and GRPR as imaging targets in locally recurrent prostate
Rybalov M, Ananias HJ, Hoving HD, van der Poel HG, Rosati S, de Jong IJ. Int J Mol Sci. 2014 Apr 10;15(4):6046-61.
Application of (99m)Technetium-HYNIC(tricine/TPPTS)-Aca-Bombesin(7-14) SPECT/CT - Application of (99m)Technetium-HYNIC(tricine/TPPTS)-Aca-Bombesin(7-14) SPECT/CT
Ananias HJ, Yu Z, Hoving HD, Rosati S, Dierckx RA, Wang F, Yan Y, Chen X, Pruim J, Lub-de Hooge MN, Helfrich W, Elsinga PH, de Jong IJ. Nucl Med Biol. 2013 Oct;40(7):933-8.