Anti-GSTM3 antibody
| Name | Anti-GSTM3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab74749 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Rabbit, Horse, Bovine, Pig |
| Antigen | A synthetic peptide corresponding to a region of human GSTM3 |
| Description | Rabbit Polyclonal |
| Gene | GSTM3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab74749 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74749 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-GSTM3 antibody (ab74749) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
Product References
Proteomic analysis of gastric cancer and immunoblot validation of potential - Proteomic analysis of gastric cancer and immunoblot validation of potential
Kocevar N, Odreman F, Vindigni A, Grazio SF, Komel R. World J Gastroenterol. 2012 Mar 21;18(11):1216-28.
Method to isolate polyribosomal mRNA from scarce samples such as mammalian - Method to isolate polyribosomal mRNA from scarce samples such as mammalian
Scantland S, Grenon JP, Desrochers MH, Sirard MA, Khandjian EW, Robert C. BMC Dev Biol. 2011 Feb 15;11:8.