
Anti-GTF2A2 antibody (ab76962) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilutiondeveloped using the ECL technique

All lanes : Anti-GTF2A2 antibody (ab76962) at 5 µg/mlLane 1 : GTF2A2 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilutiondeveloped using the ECL technique

Detection limit for ab76962 is ~0.3ng/ml as a capture antibody.