All lanes : Anti-GTF2H2C antibody - C-terminal (ab170429) at 1/100 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : NCI-H460 cell lysateLane 4 : NCI-H292 cell lysateLane 5 : A2058 cell lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling GTF2H2 with ab170429 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of NCI-H460 cells labeling GTF2H2 with ab170429 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).