ChIP analysis using ab89113 binding GTF3C2 in iPS derived from Human neuronal. Cells were cross-linked for 10 minutes with formaldehyde. Samples were incubated with primary antibody(1/100) for 16 hours at 4°C. Protein binding was detected using real-time PCR.Positive control: 7SL RNA geneNegative Control: GAPDH coding region. See Abreview
Anti-GTF3C2 antibody - ChIP Grade (ab89113) at 1 µg/ml + HeLa cell lysate at 50 µg
All lanes : Anti-GTF3C2 antibody - ChIP Grade (ab89113) at 1 µg/mlLane 1 : GTF3C2 transfected 293T cell line lysate Lane 2 : Non-transfected lysateLysates/proteins at 25 µg per lane.
ab89113, at 10 µg/ml staining GTF3C2 in HeLa cells by Immunofluorescence.